TRIC-seq Technology
Total RNA Interaction Capture (TRIC-seq) is a genetics-free, in situ proximity-ligation technology developed by our lab to map the modular architecture of the bacterial RNA-RNA interactome.
Preserving cellular context to uncover tertiary RNA structures and regulatory hubs.
Unlike traditional methodologies (like RIL-seq or CLASH) that require genetic engineering or targeting specific RNA-binding proteins, TRIC-seq operates genetics-free. By executing in situ crosslinking and proximity ligation directly inside native cells, it maps intramolecular and intermolecular contacts transcriptome-wide.
Using this technology, our lab has mapped comprehensive modular interactomes in E. coli and other bacteria, identifying unexpected regulatory hubs (such as 16S rRNA extensions contacting stress-response 5′ UTRs) and discovering stress-induced co-aggregation zones (analogous to eukaryotic stress granules).
Genetics-Free & In Situ
Preserves native state. Applicable to diverse environmental or clinical pathogens.
Intra & Intermolecular Mapping
Identifies both physical RNA folds and regulatory sRNA-mRNA networks.
